Imaging Notes for
Last Updated by
Animal
| Label | Species | Strain | Date of Birth | Age | Sex | Genotype | Notes |
|---|---|---|---|---|---|---|---|
| Rat | Mouse | M | F | Wild | Het | KO | |||||
| Rat | Mouse | M | F | Wild | Het | KO |
Virus
| Label | Serotype | Titer (vg/mL) | Rate (uL/min) | Volume (uL) | Notes |
|---|---|---|---|---|---|
| 1.8 x 10^13^ | |||||
| 1.8 x 10^13^ |
Injection
| Date | Age | Type | Site | Coordinates | Notes |
|---|---|---|---|---|---|
| | - | ICV | Local | Lateral ventricle | VB | RT | ML: mm; AP: mm; DV: mm | ||
| | - | ICV | Local | Lateral ventricle | VB | RT | ML: mm; AP: mm; DV: mm |
Euthanization
| Drug | Volume (mL) | Perfusion | Notes |
|---|---|---|---|
| Euthasol | 0.15 | transcardial | |
| Euthasol | 0.05 | transcardial |
Solutions
| Solution | Ingredients | Osmolality (mmol/kg) | Notes |
|---|---|---|---|
| NMDG | Regular NMDG | ||
| aCSF | Regular aCSF | ||
| drug | aCSF + () |
Slicing
| Plane | Thickness (um) | Speed (mm/s) | Slice Condition | Notes |
|---|---|---|---|---|
| axial | 400 | 0.04 | ice/dry ice mix |
Incubation
| Incubation Temperature | Incubation Time (min) | End Time | Notes |
|---|---|---|---|
| degrees Celsius | 12 | HH:MM |
Acquisition Protocol
| Name | Protocol | Mag | Laser | Duration (ms) | Exposure (ms) | Interval (ms) | Bin Factor | Rate (Hz) | Sweeps | Notes |
|---|---|---|---|---|---|---|---|---|---|---|
| Acq1 | markStim2 | 10x | 488 | 100 | 2x2 | |||||
| Acq2 | markStim2 | 5x | 488 | 100 | 2x2 |
Stimulation Protocol
| Name | Location | Rate | Delay (s) | Duration (ms) | Amplitude (V) | Notes |
|---|---|---|---|---|---|---|
| Same as Acq | ||||||
| Same as Acq |
Slices
| Number | Animal | Depth from LV (um) | Hemisphere | Notes |
|---|---|---|---|---|
| 1 | ~200 | |||
| 2 | ~600 |
Imaging Data
| File Name | Slice | Phase | Acq Protocol | Stim Protocol | Notes |
|---|---|---|---|---|---|
| ___ | |||||
Slice Brightfield Images
Slice 1:
Slice 2:
Slice 3:
Slice 4:
Slice 5:
Slice 6:
Slice 8:
Slice 9:
