Description

• Summary:
  
  • image/stimulate → take a break → repeat
    
• Details
  
  • Image for a set duration (e.g. 10s)
    
    • During the imaging, deliver 2 TTL pulses with the Grass S88 Stimulator:
      
      • The 1st TTL pulse drives the acquisition.
        
      • The 2nd TTL pulse drives the voltage stimulator.
        
  • Pause for set duration (e.g. 30s)
    
    • During the pause, don't image (i.e. no laser)
      
  • Repeat (e.g. 10 times)
    
    • Set the 1st TTL pulse to Repeat on the Grass S88 Stimulator
      
      

Implementation

• Summary
  
  • define imaging → define TTL pulse → define pause → define repeat → start
    
• Details
  
  • Define the imaging
    
    • In Slidebook, click on the Home tab, then hit Capture
      
      • Define the parameters of the capture
        
        • Exposure time, # of timepoints, interval, camera, etc
          
        • When happy, go to the Capture Settings pull down in the top, left of the Capture GUI
          
  • Define the TTL pulse
    
    • Plug in Grass Stimulator to TRIG/IN1 on the back of the TTL box.
      
    • Push the Advanced button below Capture Settings in the GUI
      
      • Under the General tab, and then within Captured Image Updates, select When Possible
        
      • Under the Synchronization tab, define your TTL pulse
        
      • Define when/how often in the top portion
        
      • Define TTL pulse duration in the bottom portion
        
    • Hit OK
      
  • Define the pause
    
    • The trick to defining the pause is to set up a dummy capture
      
      • 3i's Mike Muntifering set up an Orca Flash dummy setting called Flash Dummy
        
      • Flash Dummy can be selected from the Filter Set pull down within the Capture GUI
        
    • Select Flash Dummy and define a fake exposure time and # of points that will collectively achieve the total duration of your desired pause
      
    • Save the dummy capture settings in the Capture Settings pull down (upper left of Capture)
      
  • Define the repeat
    
    • Under the Special Capture tab, select Rapid 2D Capture
      
      • Select the 6D tab
        
        • Add the Capture script within the Capture Script pull down menu (top middle)
          
        • Hit the Set button on the left top-ish
          
        • Add the Pause script within the same Capture Script pull down menu
          
    • Under the Special Capture tab, select 6D Multicapture
      
      • Within the GUI, you should see the two scripts you selected above
        
      • Select Sequential within the Capture Type pull down (upper left)
        
      • Select the number of times you want to repeat within Repeat box
        
  • Hit Start