Patching Notes for

Last Updated by

Animal

Species Strain Date of Birth Age Sex Genotype Notes
Rat|Mouse | - M | F Wild | Het | KO

Euthanization

Drug Volume (mL) Perfusion Notes
Euthasol 0.04 transcardial

Solutions

Solution Ingredients Osmolality (mmol/kg) Notes
aCSF Regular aCSF
NMDG Regular NMDG
Internal solution Potassium Gluconate

Slicing

Plane Thickness (um) Speed (mm/s) Slice Condition Notes
coronal | axial ice/dry ice mix

Incubation

Incubation Temperature (degrees Celsius) Incubation Time (min) Notes
12

Pipette settings

Glass Source Puller Model Program # Notes
Thick-walled | Thin-walled P-1000

General patch settings

Bath temperature Positive pressure (mL) Negative pressure (mL) Bath flow rate (ml/min) Notes
degrees Celsius ~ ~

Patch outcomes

Pipette # Pipette Resistance (MOhm) Pipette Offset (mV) Slice # Seal Cell ID Cell Type Notes
1
2
3
4
5
6
7
8
9
10

Cell Images

Cell A (63X):

Image of Cell A (high mag)

Cell A (10X):

Image of Cell A (low mag)