Glossary for terms used in patching notes
Last updated 2018-09-14 by Adam Lu
- B6 strain: C57BL/6NJ mice (The Jackson Laboratory)
- Euthasol: pentobarbital (390 mg/mL), phenytoin (50 mg/mL)
- Regular aCSF: potassium chloride (2.5 mM), glucose (10 mM), sodium chloride (126 mM), sodium phosphate (1.25 mM), calcium chloride (2 mM), magnesium sulfate (1 mM)
- Regular NMDG: potassium chloride (2.5 mM), N-methyl-D-glucamine (92 mM), glucose (25 mM), HEPES buffer (20 mM), sodium ascorbate (5 mM), sodium pyruvate (3 mM), thiourea (2 mM), sodium phosphate (1.2 mM), HCl (92 mM), calcium chloride (0.5 mM), magnesium sulfate (10 mM); pH = 7.3~7.4 titrated with HCl
- Potassium Gluconate Internal Solution: potassium gluconate (100 mM), potassium chloride (13 mM), calcium chloride (0.07 mM), magnesium chloride (9 mM), HEPES buffer (10 mM), EGTA (10 mM), sodium ATP (2 mM), sodium GTP (0.5 mM); pH = 7.3 titrated with KOH
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Thick-walled glass: Sutter Instrument; Item #: BF150-86-10; Borosilicate capillary glass with Filament; length: 10 cm; outer diameter: 1.5 mm; inner diameter: 0.86 mm
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Thin-walled glass: Sutter Instrument; Item #: BF150-110-10; Borosilicate capillary glass with Filament; length: 10 cm; outer diameter: 1.5 mm; inner diameter: 1.1 mm
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P-1000: Sutter Instrument
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Program 33 (Katies’ whole-cell settings): Pressure: 500; Ramp: 611; Target temperature: 70 degrees Celsius
Line Heat Pull Vel Delay 1 x1 613 0 35 1 2 x1 613 0 35 1 3 x1 613 0 35 1 4 x1 614 0 36 1 -
Program 34 (Katies’ cell-attached settings): Pressure: 500; Ramp: 638; Target temperature: 70 degrees Celsius
Line Heat Pull Vel Delay 1 x1 610 0 20 1 2 x1 610 0 20 1 3 x1 610 0 20 1 4 x1 610 0 20 1 -
Program 37 (Matt’ whole-cell settings): Pressure: 500; Ramp: 611; Target temperature: 70 degrees Celsius
Line Heat Pull Vel Delay 1 x1 613 0 35 1 2 x1 613 0 35 1 3 x1 613 0 35 1 4 x1 614 0 36 1
Settings that never change:
- Vibratome model: VT1200, Leica Biosystems
- Carbogen: 95% O2/5% CO2