Oscillations Drugs
Last Updated 2019-07-31 by Adam Lu
Stock Solutions
| Name | Mass (mg) | MW (g/mol) | Solvent | Solubility (mg/mL) | Solvent Volume (mL) | Stock Concentration (mM) |
|---|---|---|---|---|---|---|
| bicuculline | 50 | 531.82 | H20 | 10.19 | 9.402 | 10 |
| NO-711 | 25 | 386.87 | H20 | 10 | 3.231 | 20 |
| SNAP-5114 | 25 | 505.60 | DMSO | > 5 | 0.494 | 100 |
| SNAP-5114 | 50 | 519.12 | DMSO | 50.56 | 0.963 | 100 |
| CGP-55845 | 25 | 438.71 | DMSO | 5 | 5.699 | 10 |
| AK3-238 | DMSO | ~0.2 | 45 |
Aliquots
| Name | Concentration (mM) | Stock volume (uL) | DMSO volume (uL) | Aliquot volume (uL) | Dilution ratio |
|---|---|---|---|---|---|
| AK3-238 | 10 | 10 | 35 | 45 | 1:4.5 |
ACSF Preparation
| Name | Bicarbonate volume (mL) | ACSF sans bicarbonate volume (mL) | Water volume (mL) | Final volume (mL) |
|---|---|---|---|---|
| ACSF | 100 | 100 | ~800 | ~1000 |
Bicuculline + ACSF Preparation
| Name | bicuculline volume (mL) | ACSF volume (mL) | Dilution ratio | Final volume (mL) |
|---|---|---|---|---|
| bicuculline + ACSF | 0.5 | 499.5 | 1:1000 | 500 |
Bicuculline + glutamine + ACSF Preparation
| Name | bicuculline volume (uL) | glutamine mass (g) | ACSF volume (mL) | Dilution ratio | Final volume (mL) |
|---|---|---|---|---|---|
| bic-glut-ACSF-1000mL | 1000 | 0.044 | ~999 | 1:1000 | 1000 |
DMSO Control Preparation
| Name | DMSO volume (uL) | ACSF/bic-ACSF volume (mL) | Dilution ratio | Final volume (mL) |
|---|---|---|---|---|
| DMSO + ACSF | 400 | ~400 | 1:1000 | 400 |
| DMSO + bicuculline-ACSF | 400 | ~400 | 1:1000 | 400 |
| DMSO + ACSF | 5 | ~500 | 1:100000 | 500 |
| DMSO + bicuculline-ACSF | 5 | ~500 | 1:100000 | 500 |
Drug solutions
| Name | Concentration (uM) | Aliquot volume (uL) | ACSF/bic-ACSF volume (mL) | Dilution ratio | Final volume (mL) |
|---|---|---|---|---|---|
| NO-711 | 4 | 50 x 2 | ~50 | 1:5000 | 500 |
| SNAP-5114 | 100 | 50 x 2 | ~50 | 1:1000 | 50 x 2 |
| NO-711 + SNAP-5114 | 4 / 100 | 10 x 2 / 50 x 2 | ~100 | 1:5000 / 1:1000 | 50 x 2 |
| CGP-55845 | 0.1 | 5 | ~500 | 1:100000 | 500 |
| AK3-238 | 0.1 | 5 | ~500 | 1:100000 | 500 |
Drug solutions
| Name | Concentration (uM) | Mass (g) | ACSF/bic-ACSF volume (mL) | H~2~O (mL) | Final volume (mL) |
|---|---|---|---|---|---|
| metformin | 10000 | 0.662 | ~396 | ~9 | ~400 |
Aliquots (obsolete)
| Name | Concentration (mM) | NO-711 stock volume (uL) | SNAP-5114 stock volume (uL) | DMSO volume (uL) | ACSF volume (uL) | Dilution ratio |
|---|---|---|---|---|---|---|
| NO-711 | 0.2 | 10 | 0 | 0 | 990 | 1:100 |
| SNAP-5114 | 5 | 0 | 50 | 0 | 950 | 1:20 |
| NO-711 + SNAP-5114 | 0.2 / 5 | 10 | 50 | 0 | 940 | 1:100/1:20 |