Interface Rig Notes for
Last Updated by
Animal
| Label | Species | Strain | Date of Birth | Age | Sex | Genotype | Notes |
|---|---|---|---|---|---|---|---|
| Rat | Mouse | M | F | Wild | Het | KO | |||||
| Rat | Mouse | M | F | Wild | Het | KO |
Euthanization
| Drug | Volume (mL) | Perfusion | Notes |
|---|---|---|---|
| Euthasol | 0.05 | transcardial | |
| Euthasol | 0.05 | transcardial |
Solutions
| Solution | Ingredients | Osmolality (mmol/kg) | Notes |
|---|---|---|---|
| NMDG | Regular NMDG | ||
| aCSF | Regular aCSF | ||
| bicuculline | aCSF + bicuculline (10 uM) |
Slicing
| Plane | Thickness (um) | Speed (mm/s) | Slice Condition | Notes |
|---|---|---|---|---|
| axial | 400 | 0.04 | ice/dry ice mix |
Incubation
| Incubation Temperature | Incubation Time (min) | End Time | Notes |
|---|---|---|---|
| degrees Celsius | 12 | HH:MM |
Amplifier settings
| Model | Calibration | Amplification | Lo Freq | Hi Freq | Line Filter | Notes |
|---|---|---|---|---|---|---|
| P511 | uV | Hz | kHz | In |
Other settings
| Bath temperature | Bath flow rate (ml/min) | Notes |
|---|---|---|
| degrees Celsius | 2 |
Slices
| Number | Animal | Depth from LV (um) | Hemisphere | Notes |
|---|---|---|---|---|
| 1 | ~200 | |||
| 2 | ~600 |
Recorded files
| File Name | Protocol Name | Slice # | Phase # | Solution | Stim amp | Pump Status | Notes |
|---|---|---|---|---|---|---|---|
| 2020XXXX_0000-2020XXXX_0033 | 20s_1sweep | 1 | NA | V | On | ||
| 60s_1sweep | 1 | 1 | 10 V | On | Baseline | ||
| 60s_1sweep | 1 | 2 | 10 V | On | Wash-on | ||
| 60s_1sweep | 1 | 3 | 10 V | On | Wash-off | ||
Slice Images
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