Note: These files can also be found in /media/shareX/Settings_EEG/Resources on Website on fishfish

Electronics

• Making a cable

  • A Theatrical Cable Making Guide (Video)
    
    

• Mouse Atlases

  • Matt Gaidica's Online Mouse Atlas
    
  • Some Beenhakker Lab Coordinates
    
  • Matt Gaidica's Online Mouse Atlas
    
  • Some Beenhakker Lab Coordinates
    
    

• Step-by-Step Guide to Making LFP recording devices

  • Wrap wire around jig
    
    • watch video
      
  • Place taped "frets" under wire.
    
    • watch video
      
  • Cut 4 pieces of plastic that will serve as your LFP electrode holders (plastic is 0.5mm thick).
    
  • Separate the wires on the jig so that they achieve the desired spacing for your target structures.
    
    • Remember that the stereoscope is magnified 2X. Therefore, the measurements in the eyepiece micrometer is doubled (i.e. 1mm = 2mm).
      
    • Be gentle. Make small, dragging movements. Alternate ends.
      
  • Place plastic pieces onto strings (on the inside of the fret). Then glue in place with super glue.
    
    • watch video
      
  • Cut glass tube (electrode casing) to right above length required for target structure (6mm is safe).
    
    • Neuralynx tubing.
      
    • One tube per electrode (corticals don't require glass tube).
      
    • Rule of thumb: less than 2.2mm deep, no glass required.
      
  • Cut wires thrice. On each end near the peg (2X) and down the middle.
    
    • The end result is a rough, working electrode manifold.
      
    • watch video
      
    • another video
      
  • Cut small pieces of tape to hold electrodes.
    
  • Place rough, working electrode down on surface, wire side up. Then tape longer wired end down so that shorter wired end pivots up.
    
  • Slip glass tubes over each subcortical electrode - slide all the way down.
    
    • Once in place, glue use super glue to fasten tubes to plastic piece. Let dry for a few minutes.
      
    • watch video (not great until 1/2 way through)
      
  • Cut electrode/tubes to desired length to achieve target depth.
    
    • Remember 2X stereoscope magnification. Therefore, for example, if target depth is 5.5mm, then length under eyepiece micrometer should read 11mm.
      
  • Cut A-M Systems, stainless steel wire about 2-2.5cm long and make lasso/hook at the end.
    
    • This piece will serve as a holder.
      
  • Coat plastic manifold with 5 minute epoxy.
    
    • Keep tape on wires so that you can hold it while coating plastic.
      
    • You can dremel wooden end of cotton swap to make cone-shaped applicator.
      
    • watch video
      
    • Once coated (and before glue has hardened), tape manifold down, with LFP electrodes hanging over the side.
      
    • Then place loop/lasso end of A-M Systems holder onto plastic part, with handle parallel to long wires.
      
    • Let glue dry.
      
  • Strip ends of non-LFP wires (~1mm). These ends will be soldered to the DigiKey manifold.
    
  • Solder wire to appropriate ends of manifold.
    
    • watch video
      
    • and another
      
  • Clamp DigiKey manifold with hemostats. Using A-M Systems holder, twist wires 5-7 times and then situate plastic end in the middle of the DigiKey manifold.
    
    • Once in place, use small strip of tape to hold down A-M Systems holder. Tape all around edge of DigiKey manifold - will have to remove hemostats. Once around, reclamp with hemostats and place electrode down for dental cementing.
      
    • watch video
      
  • Dental cement the whole shebang.
    
    • watch video
      
  • Measure electrode resistance. Aiming for >30kOhms.
    
    
    

Cutting wires

Original picture

Example of "Fill"

Example of Resize

Example of Fit with white background color

Example of Fit floating to the left

• Cut electrode/tubes to desired length to achieve target depth.
  
• Remember 2X stereoscope magnification. Therefore, for example, if target depth is 5.5mm, then length under eyepiece micrometer should read 11mm.
  
  

 
 
 
 
 
 
 
 

Example of Fit with white background color, no image borders

Example of Fit with white background color, no image borders, no captions